SynTox is the only commercially available 3D toxicology model with real-time optical monitoring and multi-compartment, multi-cellular architecture and low reagent requirements. Other benefits of this platform are:

  • Physiologically realistic morphological, fluidic and 3D cellular conditions
  • Universal platform with architecture specific of desired organ
  • Significant reduction in cost and time
  • Robust and easy to use protocols
  • Compatible with standard analytical instruments for both on chip and off chip assays including omic methodologies for systems biology and bioinformatic analysis

SynTox 3D Toxicology Model recreates the in vivo micro-environment by emulating a histological slice operating in an in vitro Environment.

Current in vitro platforms are poor predictors of in vivo safety, efficacy and pharmacokinetics of therapeutics owing to significant difference in the test conditions compared to physiological conditions observed in vivo.

invitro-invivo

Current in vitro models routinely utilize 2D monolayers or 3D aggregates of cells under static conditions for studying drug toxicity. These models fail to reproduce in vivo physiological features such as morphological size, physiological blood flow and cellular (biological) make-up of the specific organs being investigated. Other microfluidic models employ a membrane-based top-bottom two-compartment architecture, inherently limiting key desired features such as real-time visualization and the ability to simultaneous analyze multi-cellular cultures.

SynTox 3D Model Kit Components

All the basic components required to run the SynTox assay can be purchased in a kit format. All accessories including tubing, clamps, needles and syringes are included. Starter kits will also include the pneumatic priming device (required for running SynTox assays).

Qty Description Cat#
10 SynTox Chips 102016
100ft Tygon Tubing (0.02″ ID x 0.06″ OD) 201005
25 Slide Clamps 202003
50 Blunt Tip Needles (24ga x 0.5″ long) 204002
50 1mL Syringes with Luer-Lock Tips 203004
1 SynVivo Pneumatic Primer Device (in starter kits only) 205001

 

SynTox Liver model was used to demonstrate toxicity of classical pain killer acetaminophen using different modes of interaction.

Hepatocytes were cultured in SynTox model and analyzed for functionality using standard commercially available assays. Hepatocytes formed bile-canaliculi, produced urea in increasing concentration in time dependent manner with upregulated enzyme activity under physiological fluid flow conditions.

syntox-liver1

Hepatocytes form bile-canaliculi (Figure A), secrete urea with increased production in time dependent manner (Figure B) and can be monitored in real-time for enzyme activity (Figure C). Effect of flow on hepatocytes is clearly observed with increased enzyme activity.

SynTox Toxicology Model was used for understanding of drug toxicity responses using systems biology based analysis.

Liver cells and heart cells were co-cultured with their respective endothelial cells in the SynTox microfluidic chip and treated with Doxorubicin. The cells were harvested and subjected to genomic analysis resulting in upregulated and downregulated genes. The identified genes were used to develop systems biology based cellular pathway model for identification of targets and mechanism.

drug-toxicity-response-genomics

Genomic responses highlighting upregulated and down regulated cells in Endothelial Cells (Figure A), Hepatocytes (Figure B) and Cardiac Cells (Figure C)

Mono cultured hepatocytes or co-cultured with endothelial cells were treated with acetaminophen and their drug responses were investigated for different modes of interactions using a combination of Live/Dead and Reactive Oxygen Species (ROS) assays. Differences between vascular and direct treatment of hepatocytes was observed following drug interaction.

syntox-liver2

Viable co-cultures of endothelial cells and hepatocytes were observed in SynTox model(Figure A). C. Co-Culture of endothelial cells and liver cells were treated with a dose of acetaminophen followed by wash and continuous perfusion of fresh media. As seen from the images, the liver cells in the periphery (Figure D) are prone to death compared to the cells away from the vasculature as they take up most of the drugs. In contrast, none of the endothelial cells are showing any signs of distress indicating that they are not affected by the drug. Static treatment with drug results in maximum cell death (Figure C). Comparison of different modes of drug treatment for the same dosage and duration  (Figure D).  In contrast to static systems that over predict the toxicity responses, SynVivo reproduces the in vivo therapeutic responses.

Real-time Monitoring of Drug Diffusion and Drug Toxicity

SynTox microfluidic chip can be used to investigate drug diffusion in real-time across the vascular endothelium and the tissue cells to determine the duration for minimum or maximum exposure to the cells. The information obtained can be used to predict time dependent toxicity.

real-time-monitoring

Real-time monitoring of drug diffusion and time dependent toxicity. Drug diffusion across the endothelium-tissue interface (Figure A). Time dependent increase in apoptotic cells following chemotherapeutic treatment (Figure B). Time dependent increase in ROS intensity indicative of toxicity following chemotherapeutic treatment (Figure C).

3D TISSUE MODELS AND SERVICES-BROCHURE

SynTox 3D Model Technical Manual

SynTox 3D Model – Starter Kits

Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles and 50 1 ml syringes). Starter kits will also include the pneumatic priming device (required for running SynTox assays).

Cat#
Product Name
Literature
Price
404002
SynTox 3D Model Starter Kit

$1,260 Add to cart

Note: Does not include other required consumables such as cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.

SynTox 3D Model – Assay Kits

Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles and 50 1 ml syringes).

Cat#
Product Name
Literature
Price
404001
SynTox 3D Model Assay Kit

Note: Does not include other required consumables such as cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.

SynTox 3D Model – Chips

Purchase single chips.

Cat#
Product Name
Literature
Price
102016-STo
SynTox 3D Model Chip

Note: Does not include other required consumables such as tubing, clamps, syringes, needles, cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.