SynTumor is a 3D tissue model for real-time visualization and quantitative assessment of cell-cell and cell-drug interactions in a physiologically and morphologically realistic tumor microenvironment. The system enables  (a) circulation in the microvasculature, (b) transport across the vessel walls, and (c) delivery to the tumors. Starting with scans of vascular networks incorporated with interstitial and tissue/tumor spaces, the SynTumor 3D tissue model creates an in-vitro tumor microenvironment akin to a viable histological slice.

The SynTumor 3D Cancer model has the following benefits:

  • Side by side architecture enables quantitative real time visualization
  • Physiological leaky vasculature with engineered porous structures
  • Morphologically realistic in vivo based architecture
  • Physiologically realistic convective and diffusive transport
  • Microfluidic platform with ultra-low consumable volumes

Depending on your research needs you can select from the “idealized” or “microvascular” SynTumor 3D Cancer Model configuration.

Create a realistic 3D co-culture with real time monitoring of cell-cell interactions between tumor, stromal, vascular and immune cells.

Idealized Network Configuration

Available with 2µm or 8µm gap configuration.

Microvascular Network Configuration

Available with 2µm or 8µm gap configuration.

SynTumor 3D Model Kit Components

All the basic components required to run the SynTumor assay can be purchased in a kit format. Depending on individual research needs you can select from the “idealized” or “microvascular” configurations of the SynTumor chip. All accessories including tubing, clamps, needles and syringes are included. Starter kits will also include the pneumatic priming device (required for running SynTumor assays).

Qty Description Cat#
10 SynTumor Chips (idealized or microvascular configuration with 2µm or 8µm pores) 102004, 102012, 105007 or 105015
100ft Tygon Tubing (0.02″ ID x 0.06″ OD) 201005
25 Slide Clamps 202003
50 Blunt Tip Needles (24ga x 0.5″ long) 204002
50 1mL Syringes with Luer-Lock Tips 203004
1 SynVivo Pneumatic Primer Device (in starter kits only) 205001

SynTumor 3D Cancer Model Predicts In Vivo Drug Delivery Vehicle Responses

Endothelial cells and 3D spheroids of cervical tumor cells were co-cultured in the SynTumor model. Two different gene delivery nanopolymers were evaluated for their efficiencies to deliver GFP gene using both direct and vascular injection routes. In contrast to static well plate assays, SynTumor model was successful in predicting the in vivo responses of both the nanopolymers.

SynVivo and in vivo response show that both the nanopolymers labeled A and B transfect the core of the tumor with uniform GFP expression following direct injection. In contrast, vascular injection route in SynVivo shows uniform transfection of the tumors only for nanopolymer labeled A matching the observed results in vivo.

SynTumor Model Predicts In Vivo Therapeutic Responses

3D culture of endothelial cells and breast cancer cells were evaluated for their viability following treatment with chemotherapeutic drugs.  SynTumor model demonstrated significant differences in drug responses under static conditions compared to perfusion and diffusion based modes of interaction commonly observed in vivo.

Differences between vascular diffusion and perfusion experiments on tumor and endothelial cell viability.  Vascular flow and diffusion allows formation of necrotic core similar to in vivo.

Plot of viable cells for the two different modes of drug exposure compared to classical well plate studies. Results from well plate studies over predict the efficacy responses.

There are many areas of oncology research that can benefit by using the SynTumor model. These include (1) basic research for understanding of the tumor microenvironment comprising of cell viability, proliferation, invasion and tumor-stromal and tumor-endothelium interactions; and (2) drug delivery screening for efficacy and toxicity.

Understanding the Tumor Microenvironment

Cancer metastasis is a multi-step process that starts with the cancer cells leaving the original tumor site and migrating to distant parts of the body via the bloodstream or the lymphatic system.

This process involves complex steps, including breaking of the extracellular matrix by the metastatic tumor cells, escape into the circulatory system, adhesion to the vascular wall at remote locations, followed by migration/invasion into tissue and subsequent proliferation.

SynVivo provides the realistic microenvironment to allow real time study of these phenomena.

The tumor-endothelium co-culture mimics the tumor microenvironment and in this example captures degradation of the extracellular matrices by the aggressive tumor toward the vasculature channels, a precursor to extravasion and metastasis.

Monitor in real-time tumor cells phenotypic behavior. A metastatic tumor rapidly spread to adjacent chambers (left) while non-metastatic tumor does not (right). Inset image shows stained images highlighting elongated protrusions for metastatic tumor in contrast to localized for non-metastatic tumor.  Use this assay to screen tumor cell populations for their metastatic potential.

Drug Delivery Screening, Efficacy and Toxicity

Drugs or delivery vehicles (nanoparticles, polymers, liposomes, etc.) can be injected via the vascular channel or directly on the tumor under both static and physiological fluid flow conditions and their responses can be observed in real-time mimicking the in vivo conditions.

3D TISSUE MODELS AND SERVICES-BROCHURE

SynTumor 3D Model (idealized network) Technical Manual
SynTumor 3D Model (microvascular network) Technical Manual

SynTumor 3D Model – Starter Kits

Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles and 50 1 ml syringes). Starter kits will also include the pneumatic priming device (required for running SynTumor assays).

Cat#
Product Name
Literature
Price
403002
SynTumor 3D Model Starter Kit (idealized configuration, 2µm pores)

$1,260 Add to cart

403006
SynTumor 3D Model Starter Kit (idealized configuration, 8µm pores)

$1,260 Add to cart

403004
SynTumor 3D Model Starter Kit (microvascular configuration, 2µm pores)

$1,560 Add to cart

403008
SynTumor 3D Model Starter Kit (microvascular configuration, 8µm pores)

$1,560 Add to cart

Note: Does not include other required consumables such as cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.

SynTumor 3D Model – Assay Kits

Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles and 50 1 ml syringes).

Cat#
Product Name
Literature
Price
403001
SynTumor 3D Model Assay Kit (idealized configuration, 2µm pores)
403005
SynTumor 3D Model Assay Kit (idealized configuration, 8µm pores)
403003
SynTumor 3D Model Assay Kit (microvascular configuration, 2µm pores)

$1,290 Add to cart

403007
SynTumor 3D Model Assay Kit (microvascular configuration, 8µm pores)

$1,290 Add to cart

Note: Does not include other required consumables such as cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.

SynTumor 3D Model – Chips

Purchase single chips.

Cat#
Product Name
Literature
Price
102004-STu
SynTumor 3D Model Chip (idealized configuration, 2µm pores)
102012-STu
SynTumor 3D Model Chip (idealized configuration, 8µm pores)
105007-STu
SynTumor 3D Model Chip (microvascular configuration, 2µm pores)
105015-STu
SynTumor 3D Model Chip (microvascular configuration, 8µm pores)

Note: Does not include other required consumables such as tubing, clamps, syringes, needles, cells, media and matrix.
Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.